Composite

Part:BBa_K2066110:Design

Designed by: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss   Group: iGEM16_William_and_Mary   (2016-10-14)


pLacO1 sfGFP + LacI


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1087
    Illegal NheI site found at 1110
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 224


Design Notes

UNS 6.1 was used as a spacer. This part was created to reduce metabolic strain from maintaining two separate plasmids for the reporter and repressor.


Source

sfGFP and RBS from Lou et al. 2012, “Ribozyme-based insulator parts buffer synthetic circuits from genetic context” UNS sequences from Torella et al. 2013 (“Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly”


References